LOCUS       ISCR1-qnrB4-ampC_unit15701 bp   DNA    linear       26-OCT-2020
DEFINITION  Cronobacter sakazakii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Cronobacter sakazakii
  ORGANISM  Cronobacter sakazakii
            Unclassified.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-qnrB4-ampC_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15701)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Cronobacter sakazakii"
                     /mol_type="genomic DNA"
     mobile_element  1..15701
                     /gene="ISCR1-qnrB4-ampC unit"
                     /product="Putative resistance unit: ISCR1-qnrB4-ampC
                     unit"
     mobile_element  complement(1..2154)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   2134..2154
                     /gene="oriIS_ISCR1"
                     /product="oriIS of ISCR1"
     CDS             complement(2147..2611)
                     /gene="truncated sapC"
                     /codon_start=1
                     /transl_table=11
                     /product="Truncated Antimicrobial peptide ABC transporter
                     permease protein SapC"
                     /translation="MPYDSVYREKRAPGTLRTAWRNFYGDATAMIGLYGCAGLAILCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATLCGLLLGVVAGATHGLRSAVLNHILDTLLSIPSLLLAIIVVAWV"
     CDS             complement(2598..3563)
                     /gene="sapB"
                     /codon_start=1
                     /transl_table=11
                     /product="Antimicrobial peptide ABC transporter permease
                     protein SapB"
                     /translation="MIIFTLRRFLLLLVTLFFLTFIGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQLISEQLKEVFPATMELCILAFGFALMVGIPIGMLAGIT
                     RNKWQDRCISALALMGFSIPVFWLALLLTLLFSLTLGWLPVSGRFDLLYEVKPVTGFA
                     IIDAWISDSPWRDEMLVSAIRHMVLPVLTLSVAPTTEVIRLMRISTIEVFDQNYVKAA
                     ATRGLSRLTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVIGSLVIIVNVISDILGAMANPLKHKEWYALR"
     CDS             complement(3560..5203)
                     /gene="sapA"
                     /codon_start=1
                     /transl_table=11
                     /product="Antimicrobial peptide ABC transporter
                     periplasmic binding protein SapA"
                     /translation="MRLVLSSLIVMAGFLSGQAAAAPEQASYADIRDSGFVYCVSGQV
                     NTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKLNADDVVFTFERIFDRQHPWHNVNGSSFPYFDSLQFADNVQSV
                     RKLDNNTVEFRLNQPDASFLWHLATHYASVMSAEYAAKLAKQDRQELLDRQPVGTGPY
                     QLSENRAGQYIRLQRHEKFWRGTPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLQLWVPTSSQAWNPS
                     PLKTAELIQADMAQVGVKVDIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRP
                     MLSCAAINSQTNFAHWCNPEFDSILRKALSSQQLSSRIDAYDEAQNILARELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVSREKADEVKKP"
     CDS             complement(5504..5896)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKISFAPLLFCFLLPSAFADDNGGLKKDTAPPPAHALDEGYRGT
                     ENARIMTVEQAKTMHDGATISLRGNLIEDQGGDKFIFRDKTGSINTLIPLAVFDGRTI
                     KPDQMISINGSLDAKMQPPVVRVNKIQK"
     CDS             6028..6531
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MNREKTHFQGVKESLRFLNFESGFHKIGFICLLCIVFLASVGAF
                     SGGYLSEVTRKNTLKTTGLQYERFGRLQTEFKFKISAQKYDSVNKTLRIGGDFNKFYE
                     MENIWPQPDSMYSKGNDLYLVYNDSEAMQNFTIWLRVTPVKPGSVKSFLQLNGEPEIR
                     FRQFIYP"
     CDS             6541..6987
                     /gene="ycaP"
                     /codon_start=1
                     /transl_table=11
                     /product="Membrane protein YcaP"
                     /translation="MEMVFRASAIYLILLVVFKIAGRRALLQMTSFDLILLLIISEAT
                     QQALLGNDFSITGAMITIVTLVTIDILFGFIKKKVGGAESVLDGSPVILLDHGIPLLD
                     KMKKVDVSLEDILVAARQNQGITESSKIKYAILERNGHISVIPEEN"
     CDS             6998..7513
                     /gene="cinA"
                     /codon_start=1
                     /transl_table=11
                     /product="Competence-damaged protein CinA"
                     /translation="MNTEYNDAKTVNELTKRLANTLIDSDLTLTTAESCTGGKLAAAL
                     CAQADTAEFYDIGVITFSDRAKQKMLDVRASTLKKYSAVSEQTVSEMSVGIRQQAETD
                     ISIAISGYAGPEGGEDGTPAGTVWFAWNFRGQIITKRECFSGDCQDVIEKAVRFSLAV
                     LIEEVSAWKNK"
     CDS             7690..8598
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /translation="MTDLNKGKQTTNKTNSERFPQPPFPHQKQPFPGLAGKMQPRPDH
                     GEQSYQGSGRLNGRKVLITGGDSGIGRAVAIAYAREGADVAINYLPEEEDDAREVVEL
                     IKKAGRKVAAIPGDIRDETFCNHLVKQAVDALGGLDILVNNAGRQQFCESIEDLTTEE
                     FDATFKTNVYAMFWITKAAIPHFSRDSVIINTSSVQAYEPSEILLDYAQTKAAIVAFT
                     KSLAKQLAPKGIRVNAVAPGPYWTVLQCCGGQPQEKIEKFGANAPLGRPGQPVEIAPL
                     YVTLAAEENSYTSGQVWCSDGGTGTL"
     CDS             complement(8694..9341)
                     /gene="qnrB4"
                     /codon_start=1
                     /transl_table=11
                     /product="Fluoroquinole resistance protein QnrB4"
                     /translation="MMTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGC
                     QFYDRESQKGCNFSRANLKDAIFKSCDLSMADFRNINALGIEIRHCRAQGSDFRGASF
                     MNMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGG
                     EFSSFDWRAANVTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLLERLGIAVMG"
     CDS             complement(9421..10410)
                     /gene="pspF"
                     /codon_start=1
                     /transl_table=11
                     /product="Psp operon transcriptional activator PspF"
                     /translation="MVNFIMAGYKDNLLGEANSFIEVLEQVSRLAPLDKPVLVIGERG
                     TGKELIANRLHYLSTRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFE
                     RADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPRM
                     VSEGTFRADLLDRLAFDVVQLPPLRERQSDIMLMAEHFAIQMCREIGLPLFPGFTDEA
                     KETLLHYRWPGNIRELKNVVERSVYRHGTSDYPLNEIVIDPFRRHAAPPLAQETRHTS
                     VTLPLDLREFQQQQEKEFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
     CDS             10566..11234
                     /gene="pspA"
                     /codon_start=1
                     /transl_table=11
                     /product="Peripheral inner membrane phage-shock protein A"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     SNSARALAEKKQLSRRIEQASAQQAEWQEKAELALRKEKEDLARSALIEKQKLTDLIA
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHNFGKQKSLDQQFADLKADDEISEQLAQLKAKMQ
                     QDKQ"
     CDS             11289..11513
                     /gene="pspB"
                     /codon_start=1
                     /transl_table=11
                     /product="Peripheral inner membrane phage-shock protein B"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSSRGELAQSEQQRLV
                     ELNQDAQRMRERIQALEDILDAEHPNWRDR"
     CDS             11513..11872
                     /gene="pspC"
                     /codon_start=1
                     /transl_table=11
                     /product="Peripheral inner membrane phage-shock protein C"
                     /translation="MGGVNLNKKLWRIPQQGMVKGVCAGIAHYLDVPVKLVRILVVLS
                     IFFGLAFFTFVAYIVLTFVLDPMPDNVVSGEQQPSSGELLDAVDRELAASEKRLREME
                     RYVTSDTFTLRSRFRQL"
     CDS             11947..12111
                     /gene="pspD"
                     /codon_start=1
                     /transl_table=11
                     /product="Peripheral inner membrane phage-shock protein D"
                     /translation="MLLTALRYGPAGVAGWAVKSVARRPLKMLLALVLEPLLSRAATK
                     LSKRYSGNQP"
     CDS             complement(12300..12452)
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /translation="MLTPVPGGIDVPTQQLGEQSCNLLFSLIAGEKGLQNITVGTQIS
                     LADSLV"
     CDS             complement(12446..13267)
                     /codon_start=1
                     /transl_table=11
                     /product="Large extracellular alpha-helical protein"
                     /translation="MDVLRFILRLPFILLRLAARSLVYLFTLLGFLLRPFTGRIRWAV
                     PGWVTFAGNQLARLERGGNRYPKTISALLLLTAAVAAGSYYTWHWYQNKPKPVDVAPL
                     VVQDISASVQRPSAVNYNRDDNSAQIVVVTFSRSAAPVTLIGKPVTAGITLTPAMEGE
                     WQWRNDRKLVFTAKKTFPMGKTYTVDMDAKTLLAPQVALTEKQKTFTTPEFYYRGGRA
                     EFYQDPQDPMKKHAIIGLTFNAPADVKNLESRLSMTRDGKPVPYTVTVMNCCHLC"
     CDS             complement(13462..14601)
                     /gene="ampC"
                     /codon_start=1
                     /transl_table=11
                     /product="Class C beta-lactamase DHA-1"
                     /translation="MKKSLSATLISALLAFSAPGFSAADNVAAVVDSTIKPLMAQQDI
                     PGMAVAVSVKGKPYYFNYGFADIQAKQPVTENTLFELGSVSKTFTGVLGAVSVAKKEM
                     ALNDPAAKYQPELALPQWKGITLLDLATYTAGGLPLQVPDAVKSRADLLNFYQQWQPS
                     RKPGDMRLYANSSIGLFGALTANAAGMPYEQLLTARILAPLGLSHTFITVPESAQSQY
                     AYGYKNKKPVRVSPGQLDAESYGVKSASKDMLRWAEMNMEPSRAGNADLEMAMYLAQT
                     RYYKTAAINQGLGWEMYDWPQQKDMIINGVTNEVALQPHPVTDNQVQPYNRASWVHKT
                     GATTGFGAYVAFIPEKQVAIVILANKNYPNTERVKAAQAILSALE"
     CDS             14712..15587
                     /gene="ampR"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family blaDHA-1 regulator"
                     /translation="MVRRYLPLNPLRAFEAAARHLSFTRAAIELNVTHAAVSQQVRAL
                     EEQLGCVLFTRVSRGLVLTHEGEGLLPVLNEAFDRIADTLECFSHGQFRERVKVGAVG
                     TFAAGWLLPRLAGFYDSHPHIDLHISTHNNHVDPAAEGHDYTIRFGNGAWHESDAELI
                     FSAPHAPLCSPAIAEQLQQPDDVHRFTLLRSFRRDEWSRWLDCAGGTPPSPSQPVMVF
                     DTSLAMAEAAQLGAGVAIAPVCMFSRLLQSGALVQPFAAEITLGGYWLTRLQSRTETP
                     AMQQFARWLLNTAAA"
BASE COUNT     3979 a   3941 c   4087 g   3694 t
ORIGIN
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat acccaagcca
     2161 ccacaataat cgccagcaac agtgaaggga tcgacagcag ggtgtccaga atgtgattca
     2221 gcaccgcgga gcgcaaaccg tgtgttgccc cggcaaccac gccaagcaat aagccacaca
     2281 acgtggcggc aagggtgacg acaaacgcac cgcccacggt cggggccgca ccgctaagca
     2341 ggcggcttaa aacatcacgc cccaggtcat cagtcccgag gaagaaagag acttcgccat
     2401 agcgtgacca tgacggcggc aaaagctggt agccaagaaa ctgctggtcg atgccataag
     2461 gcgcgaacca accaccaaaa atacacagaa ttgccagtcc agcacagcca tacaggccga
     2521 tcattgcggt tgcatcgccg taaaaattgc gccatgcggt gcgcagcgta ccaggcgcgc
     2581 gtttctcgcg atagacgcta tcgtaaggca taccattcct tatgtttcag cgggttcgcc
     2641 atagcaccca aaatatcaga gatcacgtta acaatgataa ccagtgagcc aatcaccatc
     2701 acacccgccg aaatcgccgc gtaatcctgc tggcgaatcg cgttaatcag ccaacggcca
     2761 agacctggcc agctaaagac catctcggtg atcatcgcca gcgtcagcat ggtggaaaac
     2821 tgtaacccca ggcgcgggat aaccggtggg agagcgttgt gcagtacgtg acggcgtaaa
     2881 atcgttaacc gtgacagacc acgagtcgcc gccgctttga catagttttg atcaaagacc
     2941 tcaatagtgc tgatacgcat cagacgaata acttccgtcg tcggtgccac ggagagcgtt
     3001 aacaccggaa gcaccatatg gcggatggca ctgaccagca tttcatcgcg ccagggcgag
     3061 tcggagatcc aggcgtcaat aatggcaaac ccggttacgg gttttacttc ataaagcaga
     3121 tcaaaacgcc ctgatacggg taaccagccg agggttagtg agaatagcaa cgtcagcagc
     3181 agcgccagcc aaaaaaccgg aatagagaag cccatcagag ccaatgcgct gatgcaacga
     3241 tcctgccatt tattacgcgt tatgcccgcc aacatgccga tggggatccc gaccattagt
     3301 gcaaaaccaa atgccagaat gcacagttcc attgttgccg ggaacacctc tttcagttgt
     3361 tctgagatca actggccgtt aatactggac acaccaaaat cccagtgaat caggccattg
     3421 aaccagaata cccaagcgtt ccacagcgat gcgccctgta acggcgcatg gggggtgaaa
     3481 taactcagac tgaagccaat aaaggtcagg aagaacaaag tgaccagcaa taataaaaat
     3541 cggcgtaggg tgaagataat catggttttt tcacctcgtc agccttttcc ctggaaaccc
     3601 cggcaaaaga agcgttgccg aacggactga gcaccagccc tttgatgtca tagcggtaag
     3661 cctgcaaacg gagcgaagag gccagaggca ggatcggcaa ctcgcgcgca agaatatttt
     3721 gcgcttcgtc gtaggcatca attcgcgagg agagttgttg cgaagagagg gctttgcgca
     3781 aaatgctgtc aaactcggga ttgcaccagt gggcaaaatt ggtctgcgag ttaatggcgg
     3841 cacagctaag catcggtcgg aaaaagctgt caggatcgtt actgtccgtt gcccagccgg
     3901 acaacgttaa atcgtggttc atatccatca gacgggcctc ctgaaaacgg ccctccaccg
     3961 gaacgatatc cactttcaca cccacctgag ccatatcagc ctgaatcagc tctgcggttt
     4021 tcagcggact ggggttccag gcctgagagc tggtaggtac ccagagttgt aacgtcaggt
     4081 tttccagccc cagcgctttt aactgctcgc gcgattttgc cgggttgtac tcggtaattt
     4141 tagcttcgtt gtcataagcc cacgaggcgc gaggcaaaat agatgccgcg gtttccgccg
     4201 taccgtagta aatagattgc attaaccgtt ggttgttaat ggccagcgct agcgcatgtc
     4261 gtacggcagg gttgttcaac ggcggtttat ccgtgttgaa cgccagatag gcgatattca
     4321 tccccggacg cagcgtcagg cgcaggcgcg ggtcatcacg cagtatgctg agctggctgg
     4381 cggcaggcca tgccaggacg tcgcactcgc cggtcagtaa tttcgataag cgtccggtcc
     4441 cgccagagcc gagatccacc acgacctgcg gcatcaaggg ggtaccacgc cagaattttt
     4501 catgccgttg taggcgaata tattggcctg cacgattttc tgaaagttgg tacgggcctg
     4561 tgccgacggg ttgtctgtcg agtaattcct ggcgatcctg ttttgctaat ttggcggcat
     4621 attcggccga catcacagag gcataatgtg tggcaaggtg ccacaagaag gaggcgtcgg
     4681 gttgatttaa gcgaaattca acggtgttgt tgtccagttt acgcacgctt tggacgttat
     4741 cggcgaactg caggctatca aaataaggaa aactgctacc attgacatta tgccacggat
     4801 gctggcgatc aaagatgcgc tcgaaggtaa ataccacatc gtccgcattt agtttgcggg
     4861 tgggcgtgaa ccaggcagtc ttttgaaacg gaacatcgcg tcgtaaatgg aagcggtagg
     4921 ttgcgccgtt atctaatact tcccagcttt cagccagttc tggcaccagg cgataggtgt
     4981 agggatcgac atcaagcagt cggtcataca attgggccgc taatgtgtct acgatgagac
     5041 cgctgcttgc tttttgtgga ttgaacgtat tgacctgccc gctaacgcaa tagacaaagc
     5101 cactgtcacg aatatcagcg tacgaggcct gctcaggcgc agctgcagcc tgaccactca
     5161 gaaatccagc catcacgatc agagatgata aaaccaggcg cataatttta aagggttata
     5221 tataaagaag ctatcttact aatacttaat gacatttgcc attaccgttt gtttttgggg
     5281 cagtggggtt gataaccgcg aattcgacat cgcgtactgg tcaaaattca tacccgctat
     5341 ccactttgca tatactcttg tagctatctt agcattttca tggcccctct gactcgctta
     5401 aacgctggat ttgctgccag agagattatg tgaggtgtct gacggggaaa atgaatctgt
     5461 tgtgtgggag gcgggaagag tgaatttctt cccggctgag gtattacttc tggattttat
     5521 taacgcgcac caccggcggt tgcattttag cgtcaaggct accgttaatg ctgatcattt
     5581 gatcgggttt tatcgttcgg ccatcaaaga ccgcaagcgg gattagcgta ttgatactgc
     5641 cggttttgtc acggaagata aacttatctc ctccctgatc ctcaatgaga ttacctcgta
     5701 aagagatggt cgcaccgtca tgcatggttt tagcttgttc gacggtcatg attcttgcat
     5761 tttcagtgcc gcgatagcct tcatccagtg catgggcagg tggcggggcg gtatcttttt
     5821 tcaatccgcc attatcatcg gcaaacgctg atggcaataa aaaacagaac agtaaggggg
     5881 cgaacgatat tttcatagag cctccattga aattgatttg acttattaag tttggtagct
     5941 atttattaat ctggcgagtg ggataagtct caaattgtaa tttttcaata aaacaacggc
     6001 ttggtgttat ataagcgagg cggggagatg aacagagaga aaacccattt tcaaggagta
     6061 aaagagagcc tgcgttttct gaattttgaa tctggctttc acaagattgg ttttatctgt
     6121 ttgctctgca ttgtttttct tgcttcggtg ggagcgtttt ccggcggata tttaagtgag
     6181 gtgacgagaa aaaacacctt aaagacaaca gggttacagt acgaaaggtt tgggcgcttg
     6241 caaacggaat ttaaatttaa aatctctgca caaaagtatg attctgtgaa caagacgttg
     6301 cgcattggcg gcgatttcaa caaattttat gaaatggaaa atatttggcc gcagccagac
     6361 agcatgtaca gcaaggggaa cgatctttac ctcgtctaca atgattctga agcaatgcag
     6421 aactttacta tctggctgcg ggttacgccc gtgaagccag gaagcgtaaa gagttttctc
     6481 cagctaaatg gcgaacctga aatccgcttc agacaattta tttatcccta ggaggtgagt
     6541 atggagatgg tcttccgggc atccgccatt taccttattt tgctggtggt tttcaaaata
     6601 gcaggtcgcc gggcattatt acaaatgacc agcttcgatc tgattttatt actgattatc
     6661 agtgaagcaa ctcaacaagc cttgttgggt aatgattttt cgataaccgg cgccatgatc
     6721 acgattgtca ctctggttac tattgatatt ctgttcggtt tcattaaaaa aaaggtgggt
     6781 ggggccgaat ccgtgctgga tggttccccg gttatcctgc ttgatcacgg gatcccttta
     6841 ttagataaaa tgaagaaagt ggatgtctct cttgaagata tattagtcgc tgcgcgacaa
     6901 aatcagggga ttaccgaatc aagtaaaatc aagtatgcca ttctggaacg caacggacat
     6961 atttctgtca ttcctgaaga gaattagcga ggcggtaatg aatacggagt acaatgacgc
     7021 aaaaacagtc aatgaactga caaaacggct tgcaaacacg ctcattgata gcgatttaac
     7081 gttgaccacc gcagaatcct gtacaggagg aaagctggcc gcggcgcttt gcgcacaggc
     7141 ggataccgca gaattttatg atatcggcgt tataaccttt agcgatcgcg ccaagcagaa
     7201 aatgctcgac gtgcgggcca gtacgctgaa gaaatatagc gccgttagtg agcaaacggt
     7261 cagtgagatg tctgtaggaa tacgtcagca agccgaaacc gatatcagta tagccatcag
     7321 cggatatgca gggccggagg gtggggagga tggtactccc gcaggaaccg tgtggtttgc
     7381 gtggaatttc cgcggacaga taataacaaa acgtgagtgt ttttccgggg attgccagga
     7441 cgtcattgaa aaggcggtac gttttagcct ggcggtattg attgaagaag tgtcagcctg
     7501 gaagaacaaa taaataaacc aggacttatc ctaggaaata tttcaatgct atgtgaagtt
     7561 atcgtcgtga gagccaggtt aatattagga gaaaattaag tggtaggtaa attataaaga
     7621 ccatgtcatt cattttgttc tacgcttatt agtctttgcg atgaatgatg actcacgaga
     7681 ggtgattgaa tgactgacct aaataaagga aaacagacca ccaataaaac taattcagag
     7741 cgttttccgc agccgccttt tccacatcaa aaacaacctt ttccggggct cgccggaaaa
     7801 atgcaaccgc gccccgatca tggcgaacaa agctatcagg gaagcggcag gttaaatggt
     7861 cgcaaggtat taattaccgg tggtgattca ggtattgggc gtgctgttgc cattgcctat
     7921 gcccgcgaag gcgccgatgt cgcgattaat tatttacctg aagaagagga cgatgctcgt
     7981 gaggttgttg agttgataaa aaaagctggg agaaaagttg cggcgattcc cggcgatatt
     8041 cgtgacgaaa ctttttgtaa ccatctggta aaacaggcgg tagacgcgct gggagggttg
     8101 gatatcctgg tgaataacgc aggccgtcag caattttgtg aatcgatcga agacctcacc
     8161 acagaagaat ttgatgcgac attcaagacc aatgtctacg ccatgttttg gatcaccaag
     8221 gcggctattc cccatttctc ccgagacagc gtgattatta acacctcatc ggtacaggct
     8281 tatgagccga gtgagatcct gcttgattat gctcagacca aggcggcgat agtggcattt
     8341 actaaatcgc tggcgaagca actggcccct aaagggatcc gtgtaaatgc tgtcgcgcct
     8401 gggccgtact ggacggtatt gcagtgctgc ggcggccaac cacaggaaaa aattgagaaa
     8461 tttggtgcaa atgcgccgct tggtcgccct ggccaaccgg tagaaattgc tccgctgtat
     8521 gtcactttag cggctgaaga gaacagctat acatccggtc aggtatggtg ttctgacggg
     8581 gggaccggaa ccctctgacg tttatgcccc cgaagtcgaa acgtctgagg gggaaagcca
     8641 gcccggacaa tattgtataa gggcaggggg cggcatatac cgctccctga acattaaccc
     8701 atgacagcga taccaagacg ttccaggagc aacgatgcct ggtagctgtc cagtttgacg
     8761 ccttgcaaat caaccccgcg gatatctaaa tcgcccagtt ccgaattggt caaatcacag
     8821 tgcgtaacgt ttgctgctcg ccagtcgaag gatgaaaact cgccgccaga gaggtctgat
     8881 ccactgaacg ttgcgcccag cacctgagta cccatccagc ggttttccca cagctcgcac
     8941 ttttccagta cgacttttga aaagttggcg tagcttaagt tggtattggt gatataggcg
     9001 ctacaaaacc aggtgcgggt ggtgatcata ttcataaaac ttgcgccgcg aaaatctgac
     9061 ccttgtgccc ggcagtggcg aatttcgatt cccagcgcat tgatattcct gaaatcagcc
     9121 atggagagat cacaactttt gaaaatggca tctttcaggt tagcgcgact aaaattacat
     9181 cctttctgac tttctcgatc ataaaactgg cagccaataa attcagtgcc gctaaggtcg
     9241 gcacccgaaa aatcacagtt gaaaaatgtg ctattttcaa ctttttcacc ggtgaacctg
     9301 tttctgtcaa ttttttcgcc aactaacgcc agagtcatca tgattaacct gtttttttat
     9361 acagtgattg caacatggta aactgtttta actctcgcgt ccaacgtctg cccctgcacg
     9421 ctaaatttgg tgtttcttca gtaatgcgcg gagttgatgg taggtcagac ctaataattc
     9481 agcggctttt ttctgattaa atttcgcctg ctgtaagctg gtttgcaaaa attccttctc
     9541 ctgctgctgt tggaattcac gcagatccag cggtagggtt actgatgtat gtctggtttc
     9601 ttgcgccagc ggcggtgcgg cgtgacgcct gaacgggtca atcacaatct cattcagtgg
     9661 gtaatcgctg gtcccgtgtc ggtataccga acgctccacg acgtttttca gttcacgaat
     9721 attgcccggc caacggtagt ggaggagcgt ttctttggcc tcatcggtga acccaggaaa
     9781 caacggtaac ccgatctcac ggcacatctg aatggcaaaa tgttcagcca ttaacatgat
     9841 atcgctttga cgttcgcgta acgggggaag ttgcaccacg tcaaaggcca gacgatccag
     9901 caggtcggcg cggaacgtgc cttcactcac catgcgtggc aggtcggcgt tggttgcgca
     9961 aaccagtctt acattgacct gcagaggttg gctaccgcct acgcgttcca gctctccgta
    10021 ctcaataacg cgcagcagtt tttcctgtac cagcatcggg gcggttgcca gttcatcaag
    10081 aaacagtgtg ccgccatcgg cgcgctcgaa ccgacctggg tgacgtttct gcgcgccggt
    10141 aaaggcgccc gcttcgtgcc caaagagttc ggaatcaagt aaattttcgt tgagcgcggc
    10201 gcagttaagt gaaataaacg ggccttgcca acgggttgag aggtagtgca ggcggttggc
    10261 gataagctct ttccctgttc ctcgttcccc aatgaccagc acaggtttat ccagcggcgc
    10321 gagacgagaa acctgctcca gcacttcaat aaagctgttc gcttcgccca gcagattatc
    10381 tttgtatcct gccatgatga aattcaccat ttgttagtgt tattcaccaa tttaccctat
    10441 ttatcccgac aggtaaaatt agttattttc aaatatttca atgaattaaa aagttggcat
    10501 gcgaagtgca ttagaacagc agcagggcat tgcccgatat cagaacagta agtgaggatt
    10561 acattatggg tattttttct cgttttgccg acatcgtgaa cgccaacatt aatgcgttgc
    10621 tggaaaaggc tgaagatccg cagaagctgg tgcggttgat gatccaggaa atggaagaca
    10681 cgctggttga ggtgcgctcc aactccgcac gcgcgctggc agaaaagaaa cagttatcgc
    10741 gtcgtattga acaggccagc gcccaacagg cagagtggca ggaaaaagca gagctggcgc
    10801 tgcgtaaaga aaaagaagat ttggcgcgtt ctgcgttaat cgaaaagcag aaactgactg
    10861 acctgattgc ctccctggag catgaggtga cgctggttga cgatacgctg gcgcgtatga
    10921 aaaaagagat cggtgaactg gaaaacaaac tcagtgaaac ccgtgctcgt cagcaggcat
    10981 tgatgttgcg ccatcaggcg gcgagttctt cccgtgatgt tcgtcgtcaa ctggacagcg
    11041 gcaaactgga tgaagcgatg gctcgctttg aatcttttga gcgtcgtatc gatcaaatgg
    11101 aagctgaagc tgaaagccat aacttcggta agcagaagtc tttggatcag caatttgctg
    11161 acctgaaagc agatgatgaa atcagcgagc agttggcgca attgaaagcc aaaatgcagc
    11221 aagacaagca ataataatat ctggcggtgc ccgaacgcgc cgccgctcat cacctgtaag
    11281 gagtactcat gagcgcgcta tttctggcca tcccgttaac catctttgtg ctgttcgtct
    11341 taccgatttg gctctggctg cattacagca atcgttccag tcgcggcgag ctggcgcaaa
    11401 gtgaacaaca gcgtctggta gaacttaacc aggacgcaca gcggatgcgt gagcgcattc
    11461 aagcgctgga agacatcctt gatgctgaac atccaaactg gagggaccgc taatgggggg
    11521 cgttaatctg aataaaaaac tgtggcgtat accgcagcag ggcatggtga aaggggtttg
    11581 cgcgggtatt gcgcattatc tcgatgttcc tgtgaaactg gtgcggatcc tggtggtgct
    11641 gtccatcttt tttggtctgg cctttttcac ctttgtcgcc tacatcgtac tgacgtttgt
    11701 gttggatccg atgccagaca acgtggtttc cggagaacag cagccatcaa gcggtgagct
    11761 attggatgct gtcgatcgtg aactggctgc aagtgaaaag cgtttgcgtg agatggagcg
    11821 ttatgtaacg tctgatactt ttacattacg cagccgcttc cgccaattgt gaggttagaa
    11881 atgaataatc gctggcaacg tgccgggcaa agggttaagc cgggcattaa aatagcaggt
    11941 aagctggtgc tactgactgc gctgcgctat ggcccggctg gggtagcagg gtgggcggtg
    12001 aagtccgtgg cccgacgtcc gctaaaaatg ttgctggcgt tagtattgga acctttgttg
    12061 agccgggcag cgacaaaatt gtcgaaacgc tattcaggta atcaaccctg atatccgaaa
    12121 caacaaaagc aagcaacatt tagtcaacat tcccccagtt aatttgcggc agagtagtct
    12181 cttgtgtcat gggatcgcta ccaatagaaa tgaaggtgaa agcgacagac tccattatgc
    12241 aggtatcggt gtgaattacg cattctaaaa tgcactgggg cattgttatg ccccagtttt
    12301 tataccagtg agtcggctaa cgatatttgt gtacccacgg tgatattttg taaacctttc
    12361 tcaccggcaa tcaggctaaa taataaatta cagctttgtt cgccgagctg ctgggttggg
    12421 acatcaatgc ctcccggcac gggcgtcagc ataaatgaca acagttcatt acagtgacgg
    12481 tatacggcac cggcttgccg tcacgggtca tagacagacg cgattccaga tttttgacat
    12541 cagcgggggc attgaaggtc aggccgataa tggcgtgttt tttcatcgga tcttgcggat
    12601 cctgatagaa ctccgcccgg ccgccgcgat aataaaactc aggggtggta aaggtttttt
    12661 gtttctctgt taacgccacc tgcggagcca gcagtgtttt ggcatccata tcgacggtgt
    12721 aggttttccc catcgggaag gtttttttcg ccgtgaatac cagcttacgg tcattacgcc
    12781 actgccactc accttccatt gcgggggtca gggtaatacc ggcggtgacc ggtttaccga
    12841 tcagcgttac cggtgcggca gagcgggaga atgtcaccac cacaatctgc gctgaattat
    12901 catcacggtt ataattaacc gccgaagggc gctgcacaga cgcggaaata tcctgcacca
    12961 ccagcggcgc aacatccacc ggcttcggtt tgttctgata ccagtgccag gtgtaataac
    13021 tgcccgctgc caccgcagca gtcagtaata ataaggcaga tatggttttc ggatagcggt
    13081 tgcccccccg ctccagccgg gcaagctgat taccggcaaa ggtcacccag cccggcaccg
    13141 cccagcggat ccgtccggtg aacgggcgca gcagaaaacc cagcagagta aagagataaa
    13201 caagactgcg agccgccaga cgcagcaaaa taaaaggtaa gcggaggata aaacgcagaa
    13261 cgtccatgac aacattttcc tctgtgtgtt gagactgaca tcagtttgcc gcagcacaca
    13321 gtgaaatccg gttcagccgg aagctgtcag tgcccgatac tctcatcctc cataaaacag
    13381 cccataaagc aaattattgc ataaggaata atcttaaatt acggccccgg cgtatccgca
    13441 ggggcctgtt caggaaaaaa attattccag tgcactcaaa atagcctgtg cagctttgac
    13501 tctttcggta ttcgggtagt ttttattcgc cagaatcaca atcgccacct gtttttccgg
    13561 aataaaggcg acataggcgc cgaaaccagt tgttgcgccc gttttatgca cccaggaagc
    13621 acggttatac ggctgaacct ggttgtctgt taccggatgc ggctgcaatg cgacctcgtt
    13681 ggtcacaccg ttaatgatca tatctttctg ctgcggccag tcatacattt cccagcccag
    13741 cccctggtta atcgcggcgg ttttatagta gcgggtctgg gcgagataca ttgccatttc
    13801 cagatccgca ttaccggccc gtgacggctc catattcatt tccgcccagc gcagcatatc
    13861 ttttgaggcg gatttcacgc cgtaagattc cgcatcaagc tgtcccggcg acacgcggac
    13921 cggttttttg tttttataac cgtacgcata ctggctttgc gcactttccg gcacagtaat
    13981 aaaggtgtga gataacccca gcggtgccag gatccgtgca gtcagcaact gctcatacgg
    14041 catccccgcc gcgtttgcgg tcagagcacc aaacaggccg atactgctgt ttgcatacag
    14101 acgcatatcg cccggtttcc gggacggctg ccactgctga tagaaattca gcagatccgc
    14161 acggcttttt accgcatccg gcacctgtaa cggcagtccg cctgcggtat aggtagccag
    14221 atccagcaat gtgatcccct tccactgcgg cagagccagc tccggctggt attttgccgc
    14281 cggatcattc agcgccatct cttttttcgc cacagaaacc gcacccagca cacctgtgaa
    14341 agttttactt acagatccga gctcaaatag tgtattttca gtgaccggct gttttgcctg
    14401 aatatcggca aaaccataat tgaaataata gggcttaccc tttacggaga cggcaaccgc
    14461 catcccggga atatcctgct gtgccatcag cggtttaatg gtgctgtcca ccaccgccgc
    14521 gacattatca gcggcagaaa accccggggc ggaaaacgcc agcagagcgg aaatcagtgt
    14581 tgcagataac gattttttca tcagaattaa ccttccgtgt gtgagtaacg gcggcaagta
    14641 tcgtcagatt caccggacag aacaaacggt tatttataac aagagcctaa agaaaaactt
    14701 acaggtggat tatggtcaga cgttatctcc cccttaaccc gctgcgcgcc tttgaggccg
    14761 ccgcccgtca tctcagtttt acccgcgcgg cgattgagct gaatgtcacc catgccgccg
    14821 tcagccagca ggtcagggcg ctggaagaac aactcggctg tgtgctgttt acccgcgtct
    14881 cgcgcgggct ggtgctgacc catgaaggtg agggattact gccggtgctc aatgaggcgt
    14941 ttgaccggat tgcggatact ctggagtgtt tttctcacgg gcagttccgt gagcgggtga
    15001 aagtcggtgc ggtgggaaca tttgccgcag gctggctgct gccgcgtctg gccggattct
    15061 atgacagcca tccgcatatt gatctgcata tctccaccca taacaatcat gtggacccgg
    15121 cggcggaagg gcatgattat acgatccgtt tcggtaacgg cgcgtggcat gagtcagatg
    15181 cggaactgat tttcagtgca ccacacgctc cgctgtgctc accggccatt gcagaacagt
    15241 tacagcagcc ggatgatgtt caccgcttta ccctgctgcg ctcattccgc cgggatgaat
    15301 ggagccgctg gctggattgt gcgggcggca caccgccttc cccgtcacag ccggtaatgg
    15361 tgttcgatac ctcactggcc atggccgagg cggcacaact gggtgccggg gtagcgatcg
    15421 caccggtatg tatgttcagc cgcctgttac agtcaggcgc actggtacag ccgtttgccg
    15481 cagaaatcac cctcggcggc tactggctga cgcggttaca gtcccgtacg gaaaccccgg
    15541 ccatgcagca attcgcccgc tggctgctga atacggcggc ggcgtaaaac tcactcacct
    15601 tccaggcttt ttacccgcaa atcatcacct tcactgatgc gcagccgttc actgccgcag
    15661 tgcggacagc atccggcgtg ctgcatgatt tcggcctcac g
//
